The moment happened mid-way through the show when presenter Alexander Armstrong started asking contestant Sarah about her profession.
“I’m a self-employed dog walker,” she explained to the comedian and Pointless host.
Sarah from Poole went on to say: “I’ve got my own dog, can I say hello to him?”
Alexander, 47, seemed to be in a mischievous mood and abruptly told her: “I think not. No,” but luckily it was all in jest and he quickly told her: “No, of course you can! Of course you can!”
His answer was met with laughter from the audience as he asked her the name of her dog and fellow presenter Richard Osman gave a wave to the avid canine viewer.
“He’s called Godfrey, he always watches the show,” Sarah told Alexander and the audience.
Both Sarah and her friend Maria from Southampton made it through to the final round of tonight’s episode but luck did not seem to be on their side when they failed to get any Pointless answers.
This evening’s instalment of the BBC quiz also proved to be a unique one after it was revealed that there were four completely new pairs of contestants.
It was described as something of a rarity by Richard, 46, who was heard exclaiming: “A very weird occurrence, it doesn’t happen very often: four entirely new pairs.”
He added: “This hasn’t happened for 80 shows! It’s statically weird. You would think it would happen quite often but four entirely new pairs.
“I haven’t a got a clue what anyone’s like. They could be geniuses, it should be lots of fun.”
Pointless airs on BBC One on weekdays at 5.15.pm.